Figure 2
From: Human stem cell-derived GABAergic neurons functionally integrate into human neuronal networks
Electrophysiological properties of hdINs during maturation process over time in culture. Differential cell response (A–C) to 0–25 pA ramps of depolarizing current and (D–F) 50 pA depolarizing current pulses at different time points. (G) hdIN filled with biocytin. (H–I) RMP and Ri at different time points. (J) Increased proportion of cells with spontaneous APs (colored area) over time. (K) Maximum number of APs that cells fired in response to 500 ms current pulses. (L–O) Distribution of AP threshold (L), amplitude (M), duration (N) and afterhyperpolarization (O) at different time points. (P–R) Expanded current traces illustrating the sodium current and (T–V) the potassium current activated during voltage pulses ranging from − 90 to + 40 mV in 10 mV steps at different time points. Sodium and potassium currents were blocked by TTX (1 µM) and TTX + TEA (10 mM) respectively (black lines). Sodium (S) and potassium (W) current peak plotted against the voltage steps. Scale bar: 100 µm. Mean ± SEM. One-way ANOVA with Tukey’s post hoc test and Fisher’s exact test (25 DIV n = 15 in blue, 35 DIV n = 36 in green, and 49 DIV n = 26 in red). *p < .05; **p < .01; ***p < .001; ****p < .0001; compared to 25 DIV group. Two-way ANOVA with Tukey’s post-hoc test. #, 25DIV vs 35DIV; $, 25DIV vs 49DIV; &, 35DIV vs 49DIV (25 DIV n = 15 in blue, 35 DIV n = 28 in green, and 49 DIV n = 33 in red). AP, action potential; AHP, afterhyperpolarization; RMP, resting membrane potential; Ri, input resistance.
