Table 1 PCR condition and primer sets used for the screening of Vibrio spp.

From: Epidemiologic potentials and correlational analysis of Vibrio species and virulence toxins from water sources in greater Bushenyi districts, Uganda

Species

Primer

PCR primer sequence (5′–3′)

Amplicon size (bp)

PCR cycling condition

References

Vibrio

16S rRNA

CGGTGAAATGCGTAGAGAT

TTACTAGCGATTCCGAGTTC

663

Initial denaturation (94 °C for 5 min), denaturation (94 °C for 30 s), 35 cycles, annealing (52 °C for 30 s), extension (72 °C for 60 s), final extension (72 °C for 10 min)

44

V. parahaemolyticus

toxR

TGTACTGTTGAACGCCTAA

CACGTTCTCATACGAGTG

503

Initial denaturation (94 °C for 5 min), denaturation (94 °C for 30 s), 35 cycles, annealing (55 °C for 30 s), extension (72 °C for 60 s), final extension (72 °C for 10 min)

45

V. vulnificus

vvhA

ACTCAACTATCGTGCACG

ACACTGTTCGACTGTGAG

366

Initial denaturation (94 °C for 5 min), denaturation (94 °C for 30 s), 35 cycles, annealing (55 °C for 30 s), extension (72 °C for 30 s), final extension (72 °C for 10 min)

V. cholerae

toxR

GAAGCTGCTCATGACATC

AAGATCAGGGTGGTTATTC

275

Initial denaturation (94 °C for 10 min), denaturation (94 °C for 60 s), 35 cycles, annealing (59 °C for 60 s), extension (72 °C for 1 min), final extension (72 °C for 10 min)

46

OmpW

CACCAAGAAGGTGACTTTATTGTG

GGTTTGTCGAATTAGCTTCACC

304

V. fluvialis

toxR

GGATACGGCACTTGAGTAAGACTC

GACCAGGGCTTTGAGGTGGACGAC

217

Initial denaturation (94 °C for 5 min), denaturation (94 °C for 60 s), 35 cycles, annealing (57 °C for 60 s), extension (72 °C for 90 s), final extension (72 °C for 7 min)

47

V. alginolyticus

Vg gyrB F

Vg gyrB R

GAGAACCCGACAGAAGCGAAG

CCTAGTGCGGTGATCAGTGTTG

338

Initial denaturation (93 °C for 5 min), denaturation (92 °C for 30 s), 30 cycles, annealing (56 °C for 1 min), extension (72 °C for 1.5 min), final extension (72 °C for 7 min)

48

V. mimicus

VM-F

VM-R

CAGGTTTGCTGCACGGCGAAGA

CCTTGAAGAAGCGGTTCGTGCA

177

Initial denaturation (93 °C for 5 min), denaturation (92 °C for 30 s), 30 cycles, annealing (57 °C for 1 min), extension (72 °C for 1.5 min), final extension (72 °C for 7 min)

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