Figure 5

Effects of miRNA mimics on monkey SCE cells. (A) Quantitative RT-PCR was performed using the indicated primers to analyze monkey SCE cells transfected with miRNA mimics (miR-23a-5p mimic (23a-5p), miR-3942-5p mimic (3942-5p), miR-7515 mimic (7515)) or a miRNA positive control targeting GAPDH (control). Fold changes in the indicated mRNA expression relative to the level of the miRNA mimic control are presented (n = 4 for each group). *:p < 0.05, **:p < 0.01, ***:p < 0.001 by Dunnett’s test. VEGF: vascular endothelial growth factor, VEGFR: VEGF receptor, NRP: neuropilin, PECAM: platelet endothelial cell adhesion molecule, ACTA: α-smooth muscle actin, FN: fibronectin. (B) Western blot analysis of cell lysates after transfection with the indicated miRNA mimic. The graphs show the relative intensity (determined with Image J software) of the indicated proteins compared to β-actin, which was used as a loading control (n = 3). *:p < 0.05, **:p < 0.01 by Dunnett’s test.