Figure 3
Changes in the biology of the transplanted tumors. (A) Ki-67 labeling index. (B) Apoptotic index. (C) Microvascular density (MVD) index. (D) Tumor invasion index. The tumors of the IR/Ipsi-tumor group exhibited significantly higher proliferation, angiogenesis abilities and invasiveness compared to those of the Sham-IR/Tumor group. Tumors of the IR/Ipsi-tumor and IR/Contra-tumor group showed significantly lower rates of apoptosis compared to the Sham-IR/Tumor group. These results were expressed as mean ± SD and comparisons between groups were assessed by Student’s t-test. (E) F98 glioma xenografts were stained immunohistochemically for Ki-67 (upper panels), TUNEL (middle panels) and CD34 (lower panels) at a magnification of × 400. Tumors of the IR/Ipsi-tumor and IR/Contra-tumor group showed a significant increase in proliferative index and a significant decrease in apoptotic index compared to Sham/IR-Tumor group. Tumors of the IR/Ipsi-tumor showed a significant increase in microvascular density index compared to Sham/IR-Tumor group. Scale bars = 50 μm. (F) Hematoxylin and eosin staining of F98 tumor showed tumor rim for sham-IR/Tumor (left panels), IR/Contra-tumor (middle panels) and IR/Ipsi-tumor (right panels). The upper panel shows the image at a magnification of × 80, and the area surrounded by the square shows the image at a magnification of × 200 (lower panel). Scale bars = 100 μm.
