Figure 1
From: Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function
Morphometric analysis of TH network in d30 neurons. (A) Representative image of a maximum intensity projection of a Z-stack fluorescently labelled with Map2 and TH antibodies. Images taken using a ×25 objective, scale bar is 50 μM. Identification and quantification of parameters: (B) TH Area, (C) Map2 Area, (D) TH Branchpoints, (E) Map2 Branchpoints and (F) TH Area/Map2 Area were determined. Four biological replicates were used for the analysis with a minimum of six Z-stacks analysed by replicate. The graphs displayed in columns show data points that refer to the average data per Z-stack. Relative quantification in the patient-derived neurons of: (G) TH, (H) TUBB3, (I) GFAP and (J) DAT. Each data point refers to 4 independent biological replicates. For all statistical analyses, an ordinary one-way ANOVA was performed with Tukey’s post-hoc multiple comparison test. Graphs (B–F) were plotted as mean ± SEM; graphs (G–J) were plotted as mean ± SD. *p < 0.05, ** p < 0.01, ***p < 0.001 ****p < 0.0001.
