Figure 2
From: Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function
Multi-electrode array neuronal recording of d55 neurons. (A) Representative image showing the plate layout of recorded neurons with a heat map displaying the mean firing rate. (B) Representative example of a Waveform profile, (C) Representative example of a Spike plot, (D) Representative image of a raster plot profile, taken from the well highlighted in (A). Spike and burst metric parameters identified from the MEA recording: (E) Mean Firing Rate, (F) Number of Bursts, (G) Burst Percentage, (H) Burst Frequency, (I) Average Burst Duration, (J) Number of Spikes/Burst, (K) Average Inter-Burst Interval, and (L) Average Inter-Burst Interval (IBI) Co-efficient of Variation. Three biological replicates were used in the analysis with a minimum of six technical replicates per recording. The graphs displayed in columns show individual values that refer to the average data of each well, with each well composed of 16 electrodes. For all statistical analyses, a non-parametric Kruskal–Wallis test was performed using the Dunn’s post hoc multiple comparison test. All graphs were plotted as mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001.
