Figure 2

Select sHSP genes are induced during UPR induction. Transcript levels of the l(2)efl genes 410087b (A), 724274 (B), 410087a (C), 724367 (D), 724405 (E), 724449 (F), and 724488 (G) relative to β-actin in midgut tissue from adult bees captured at the landing board and fed sucrose solution containing tunicamycin (T, n = 8) or vehicle alone (C, n = 8) for 24 h. A schematic diagram of the colocalized l(2)efl genes in the honey bee genome is shown below for reference. Mean ± SEM is shown and represents expression values of the genes of interest calculated using the 2^(−ΔCT) method for individual bees. Statistical significance is noted as *p < 0.05, and **p < 0.01.