Figure 4

Select sHSP genes are induced during Heat-Shock. Transcript levels of the l(2)efl genes 410087b (A), 724274 (B), 410087a (C), 724367 (D), 724405 (E), 724449 (F), and 724488 (G) after heat shock relative to β-actin in midgut tissue from adult bees captured at the landing board and maintained for 4 h in cages at either 35° (n = 6) or 45 °C (n = 6). A schematic diagram of the colocalized l(2)efl genes in the honey bee genome is shown below for reference. Mean ± SEM is shown and represents expression values of the genes of interest calculated using the 2^(−ΔCT) method for individual bees. Statistical significance is noted as *p < 0.05, and **p < 0.01.