Figure 5 | Scientific Reports

Figure 5

From: A point-mutation in the C-domain of CMP-sialic acid synthetase leads to lethality of medaka due to protein insolubility

Figure 5

Kinetic analysis of WT and L304Q mdkCSS. (A) Purified recombinant enzymes used for the kinetic analysis. SDS-PAGE/CBB staining (left). Western blotting using the anti-mdkCSS antibodies (right). Full-length blots for A are presented in Supp_FigS11. (B) Lineweaver–Burk plots for the synthesis of CMP-Neu5Ac by mdkCSSs. (C) Kinetic parameters. Km, Vmax, and Vmax/Km values are expressed as average values ± standard deviations from three independent experiments (n = 3).

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