Figure 8
Molecular modeling for WT and L304Q mdkCSS. (A) Comparison of the local amino acid sequence around L-304 residue among vertebrates. Medaka, Oryzias latipes; Rainbow trout, Oncorhinchus mykiss; Frog, Xenopus tropicalis; Turtle, Mauremys reevesii; Chicken, Gullus gullus; Mouse, Mus musculus; Human, Homo sapiens. (B) Molecular modeling of the C-domains of WT and L304Q. These models are constructed by the MOE homology modeling based on the crystal structure of the C-domain of mouse CSS (PDB: 3EWI). Magnified views around L-304 residue are shown at lower panels. Two hydrogen bonds newly appear between H of NH group of the Q304 side chain and O of carbonyl group of the Q326 backbone, and between H of NH group of the Q304 backbone and O of carbonyl group of the Q326 backbone. No such interaction is not observed in WT.
