Table 1 Overview of the experimental parameters of the different THz-SAXS beamtimes.
Date | Dec 2018 | Aug 2019 | Oct 2019 | Aug 2020 |
|---|---|---|---|---|
THz source | Source II | Source II | Source I | Source II |
Frequency [THz] | 0.1–6.0 | 0.1–6.0 | 0.5 | 0.1–6.0 |
Cw/pulsed | Pulsed: 100 MHz | Pulsed:100 MHz | Cw | Pulsed: 100 MHz |
Power (average) [mW] | 0.03 | 0.06 | 1.0 | 0.06 |
Power density [mW/cm2] | 0.4 | 0.8 | 6.5 | 0.8 |
Spot size diameter (free) [mm] | 3 | 3 | 4 | 3 |
Spot size* (on sample) [mm] | 2 | 2 | 2 | 2 |
Deposited energy** [µJ] | 5 | 4 | 75 | 10 |
SAXS | ||||
X-ray energy [keV]/wavelength [nm] | 10/0.124 | 10/0.124 | 10/0.124 | 10/0.124 |
Sample-detector distance [m] | 3.0 | 4.0 | 3.0 | 3.0 |
Detector | PILATUS 6 M | PILATUS 6 M | PILATUS 6 M | PILATUS 6 M |
Exposure time [ms] | 100 | 100, 200 | 200 | 50 |
Number of frames | 50 | 50 | 250 | 50 |
Samples | Microtubules | Microtubules/BSA | BSA | BSA |
Concentration [mg/mL] | 3.5 | 3.5/4.4–5.3 | 5.8 | 2.5, 10.9 |
Sample volume [µl] | 120 | 120 | 120 | 120 |
Flow rate [µL/s] | 2 | 5 | 2 | 2 |
Dwell time (in THz beam) [s] | 0.4 | 0.2 | 0.4 | 0.4 |
THz status during SAXS | On/off | On/off | On/off/alternating | On/off |
