Table 2 Allelic richness (Ar), private allelic richness (pAr), observed heterozygosity (Ho), expected heterozygosity (H_E) and inbreeding index (Fis) were calculated independently for each population based on the genotypes of female individuals.

Location	N		Ar		pAr		Ho		H_E		Fis
Location	hIN	hOUT	hIN	hOUT	hIN	hOUT	hIN	hOUT	hIN	hOUT	hIN	hOUT
PT_VC	14	13	4.45	4.43	0.19	0.16	0.586	0.572	0.659	0.660	0.148	0.174
SP_SG2	54	49	4.29	4.32	0.11	0.10	0.532	0.524	0.671	0.672	0.217	0.229
SP_SG3	22	19	4.27	4.21	0.13	0.10	0.590	0.576	0.680	0.665	0.156	0.162
SP_MU1	15	11	4.63	4.57	0.30	0.31	0.708	0.733	0.700	0.687	0.033	− 0.01
SP_MU2	17	11	4.44	4.60	0.12	0.09	0.570	0.619	0.698	0.690	0.215	0.157
SP_SN2	19	12	4.45	4.42	0.11	0.13	0.570	0.624	0.687	0.662	0.197	0.101
SP_SN3	36	20	4.50	4.37	0.16	0.16	0.629	0.611	0.701	0.683	0.117	0.131
SP + PT	177	135	4.46	4.43	0.39	0.40	0.587	0.584	0.701	0.696	0.166	0.165
REF_BL	11	11	4.48	4.48	0.13	0.18	0.717	0.717	0.661	0.661	− 0.036	− 0.036
REF_FR	17	14	4.70	4.62	0.23	0.15	0.739	0.778	0.716	0.707	− 0.001	− 0.064
PN_EY	15	8	4.16	3.85	0.11	0.13	0.588	0.644	0.630	0.589	0.107	0.07
REF_SP80	34	33	4.69	4.66	0.36	0.37	0.498	0.488	0.710	0.706	0.314	0.326

N = population size. hIN includes all individuals while hOUT excludes hybrid individuals (morphological and mitochondrial).

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