Figure 8 | Scientific Reports

Figure 8

From: Activation of antioxidative and detoxificative systems in Brassica juncea L. plants against the toxicity of heavy metals

Figure 8

Transcriptional levels of genes encoding antioxidative (CuZnSOS—A, MnSOD—B) and detoxitative (GR1—C, γECS—D) enzymes in roots and shoots of B. juncea v. Malopolska seedlings. Plants were grown in Hoagland’s medium and treated with bimetals combinations: CuPb, CuCd, CuZn, PbZn, PbCd, and ZnCd. Metal solutions Pb(NO3)2, CuSO4, CdCl2, and ZnSO4 were applied of each at a 25 μMol concentration. Enzymes chosen for the experiment were amplified using semi-quantitative RT-PCR. Primers were designed for Arabidopsis thaliana genes: CSD1 for CuZnSOD, MSD1 for MnSOD, GR1 for GR, and γECS.

Back to article page