Table 2 Primer sequences used for qPCR in this study.

From: Climate change affects the parasitism rate and impairs the regulation of genes related to oxidative stress and ionoregulation of Colossoma macropomum

Gene

(Sequence 5′–3′)

Eff (%)

References

β-actin

F: GCTCCCCCTAGCGTAAATACT

103.22

RNA-Seq by Prado-Lima and Val42

R: TGGACAGGGAGGCCAAGAT

β-Tubulin

F: GACGTGGTGCCCAAAGATGT

100.55

R: TGGATGGTGCGCTTGGT

Nrf2

F: CTCCCAATTCAGGCAGATAC

95.38

RNA-Seq by Fé-Gonçalves et al.79

R: CACTGCTTGAACATCCAGG

sod1

F: CAGGACCACACTATAACCCC

102.47

R: CTCCCAGCAGTCACATTACC

nka α1a

F: TGCGCCTGAGGATGAGCTTT

95.4

R: CAACTGGTGGTTCTGCGCTT

Hif-1α

F: ATCAGCTACCTGCGCATG

97.51

Silva et al.80

R: CTCCATCCTCAGAAAGCAC

  1. Primers for housekeeping genes (β-actin and β-Tubulin) and primers for target genes (Nrf2, SOD1, NKA α1a and HIF-1α).
  2. Eff = Primer efficiency.
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