Table 3 Details of the primer sequences of cytokines used for quantitative SYBR Green real-time PCR amplification. The rpl-4 gene was used as reference.

From: Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter

Target gene

Primer sequence

Access number

qPCR efficiency (%)

Slope

R2

Amplicon size (bp)

Amplicon melting temperature (°C)

rpl-4

(F)CAAGAGTAACTACAACCTTC

NC_010443.5

96.79

3.401

0.994

122

75

(R)GAACTCTACGATGAATCTTC

IL-8

(F)AGGAAAAGTGGGTGCAGAAG

NM_213867.1

97.67

3.379

0.991

190

78

(R)CAACCCTATGTCTGACCAGC

IFN-γ

(F)ATTGGAAAGAGGAGAGTGAC

NM_213948.1

96.54

3.408

0.997

168

76.5

(R)CATTCAGTTTCCCAGAGCTA

IL-4

(F)AGAGCTCTATTCATGGGTCT

NM_214123.1

99.88

3.325

0.997

209

82

(R)CTTCTCCGTCGTGTCTCT

TGF-β

(F)GGATACCAACTACTGCTTCA

NM_214015.2

100.85

3.302

0.996

228

83

(R)TTGTACAGAGCCAGGACTT

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