Figure 1

Spectroscopic characterization of the T. maritima encapsulin. (A) Strategy utilized to maximize cargo loading within the encapsulin shell. (B) T. maritima encapsulin exhibits a yellow hue upon purification. For panels (C–E), the colors absorbance spectrum for WT encapsulin is in purple, and FMN is in orange, and the purified FLP cargo alone is in maroon. (C) Encapsulin spectrum has maxima and minima similar to that of FMN. Fluorescence emission after excitation at 450 nm for native samples (D) and under denaturing conditions (E).