Figure 2 | Scientific Reports

Figure 2

From: The encapsulin from Thermotoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein

Figure 2

Structural determination confirms the presence of a flavin coordinated with W90. (A) Cryo-EM structure of the T. maritima encapsulin determined at 3.3Å resolution. (B) Zoomed-in view of a portion of the density map and corresponding atomic model showing the flavin density (in yellow) next to the tryptophan at position 90 (W90, in green). (C) Absorbance spectra of WT encapsulin (purple) and W90E (blue). (D) Color difference between wildtype and mutant W90E encapsulins after purification, protein concentration identical between the two samples.

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