Figure 3 | Scientific Reports

Figure 3

From: The encapsulin from Thermotoga maritima is a flavoprotein with a symmetry matched ferritin-like cargo protein

Figure 3

Initial cryo-EM visualization of the FLP-cargo protein within the encapsulin shell. (A) Raw micrograph of the FLP-loaded encapsulin. The inset shows an expanded view of a single particle to show that multiple FLP cargo proteins are clearly visible within the encapsulin, even before processing. (B) Curated 2D class averages further showing FLP density in some classes and illustrating differences in FLP loading: ~ 2 FLPs per encapsulin (upper-left), 3–4 per encapsulin (bottom-right), as well as a small population lacking any cargo protein (bottom-left). (C) Slice through the encapsulin structure showing the shell in purple and weak, noisy FLP densities in red.

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