Figure 4
The fivefold FLP cargo sits on the fivefold axis of encapsulin. (A) Cryo-EM map resulting from symmetry expansion and focused classification, colored by local resolution, with the shell at ~ 3.5 Å resolution and the FLP cargo at ~ 7 Å. (B) Unambiguous docking of a known FLP decamer structure (PDB:5da5), with the crystal structure shown by itself in (C). The alternating blue and green coloring are for visualizing the distinct pentamer units of the FLP cargo. (D) is a 90° rotation and the atomic model for the map shown in (A). This structure shows how the FLP sits at the fivefold axis as well as the flexible linker that connects the body of the FLP protein to the C-terminal 8 residue signal sequence that connects the FLP to the encapsulin shell (E), confirming what had been identified in previous studies4.
