Figure 3 | Scientific Reports

Figure 3

From: Robust unsupervised deconvolution of linear motifs characterizes 68 protein modifications at proteome scale

Figure 3

EGF pathway inhibition analysis. (a) Enriched phosphorylation patterns identified by RoLiM. Phosphosites analysed in RoLiM lacked motifs matched to curated kinases in Olsen et al. data (see “Methods”). The abundance of each pattern (calculated as the average abundance of all phosphosites, is shown for the different experimental conditions—RPE1 cells without EGF stimulation (No serum), with EGF stimulation (EGF), and treated with 1 µm of a panel of inhibitors targeting 10 kinases in the EGF pathway. (b) A scheme of the EGF pathway showing EGFR and downstream kinases targeted with specific inhibitors in Olsen et al. Broken lines denote a series of steps involving other proteins and phosphosite abundance changes are depicted in blue (downregulation) and red (upregulation).

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