Figure 5

Plasma E2 (A) and 11KT (B) concentrations in females, early, mid- and late transitioning fish, and TP and IP males obtained across the three experiments, AI2014, SI2016 and SI2018. Each red (AI2014), yellow (SI2016) and blue (SI2018) line represents the variation in mean E2 or 11KT concentrations across groups per experiment while the black line represents the variation in overall mean concentrations for the three experiments altogether. Sample sizes: E2, F n = 36, ET n = 42, MT n = 8, LT n = 14, TP^↑ n = 17, IP n = 5; 11KT, F n = 40, ET n = 41, MT n = 9, LT n = 15, TP^↑ n = 26, IP n = 4. 11KT 11-ketestosterone, AI2014 aromatase inhibition experiment 2014, F initial phase female, E2 17β-estradiol, ET early transitioning fish, IPM initial phase male, LT late transitioning fish, MT mid-transitioning fish, SI2016 social induction experiment 2016, SI2018 social induction experiment 2018, TP terminal phase male. ^↑Both control males used throughout the experiments to create a socially inhibitory environment (SI2016: E2 n = 10, 11KT n = 10; SI2018: E2 n = 0, 11KT n = 10), and males obtained through sex change of manipulated females (SI2016: E2 n = 1, 11KT n = 1; SI2018: E2 n = 5, 11KT n = 5) were grouped altogether as TP males for the purpose of this analysis.