Figure 1 | Scientific Reports

Figure 1

From: Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells

Figure 1

Identification of a vWF receptor in S. aureus SH1000 spa. (A) Lysostaphin-released material from S. aureus SH1000 spa grown in RPMI (lane 1) and BHI (lane 2) was subjected to Far Western Blotting. The nitrocellulose membrane was probed with human vWF followed by polyclonal rabbit vWF antibody and HRP-conjugated goat anti-rabbit IgG. (B) Lysostaphin-released material from S. aureus SH1000 spa grown in RPMI and BHI was subjected to Western Immunoblotting. The nitrocellulose membrane was probed with a rabbit anti-IsdB IgG and HRP-conjugated goat anti-rabbit IgG. Molecular mass standards are indicated on the left of the panels. (C) Binding of vWF to surface proteins from S. aureus. Microtiter wells were coated with purified recombinant A regions of the indicated CWA proteins of S. aureus and incubated with human vWF. Ligand bound to the wells was detected with a polyclonal rabbit vWF antibody followed by HRP-conjugated goat anti-rabbit IgG. The data points are the means ± SD of two independent experiments. In the inset, binding of the vWF to purified recombinant IsdB NEAT1-NEAT2 protein in a Western blotting assay is shown. The full-length blots are presented in Supplementary Fig. S3.

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