Figure 2 | Scientific Reports

Figure 2

From: Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells

Figure 2

Capture of vWF by S. aureus cells. (A) S. aureus SH1000 WT and its spa or isdB mutant were grown in RPMI to stationary phase. The staphylococcal cells were incubated with purified human vWF (5 µg/ml) and bacteria-bound protein was released by extraction buffer and subjected to SDS-PAGE and Western Immunoblotting. 2 µg of purified vWF was loaded as control. The membrane was probed with a polyclonal rabbit vWF antibody followed by HRP-conjugated goat anti-rabbit IgG. Molecular masses of standard proteins are indicated on the left. The full-length blot is presented in Supplementary Fig. S3. (B) Densitometric analysis of vWF bound to S. aureus WT and its isogenic spa or isdB mutant. Statistically significant differences are indicated (*, P < 0.05; **, P < 0.01). The reported data are the mean values ± SD from three independent experiments.

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