Figure 3
Adhesion of S. aureus and L. lactis ectopically expressing IsdB to immobilized vWF. (A) Adhesion of S. aureus SH1000 WT and its isogenic isdB mutant to immobilized vWF. Microtiter wells coated with vWF were incubated with cells of S. aureus SH1000 WT and its isdB mutant obtained from cultures grown in RPMI. Bacteria bound to vWF were detected by the addition of HRP-conjugated rabbit anti-mouse IgG to the wells. Statistically significant difference is indicated (**, P < 0.01). (B) Adhesion of L. lactis expressing IsdB to immobilized vWF. Microtiter wells coated with vWF were incubated with L. lactis ectopically expressing IsdB (L. lactis pNZ8037::isdB) or L. lactis carrying the empty vector (L. lactispNZ8037). Bacteria bound to vWF were detected incubating the wells with a polyclonal rabbit anti-L. lactis IgG and HRP-conjugated goat anti-rabbit IgG. Statistically significant difference is indicated (*, P < 0.05). The data points are the means ± SD from three independent experiments, each performed in triplicate.
