Table 1 Abbreviations used are as follows: Cm^r, chloramphenicol resistance, Tc^r, tetracycline resistance.

S. aureus

SH1000 WT

Laboratory strain. rsbU ⁺ derivative of S. aureus 8325‐4

⁵³

SH1000 spa

spa::Tc^r transduced from 8325–4 spa::Tc^r

⁵⁴

SH1000 isdB

isdB gene deleted by allelic exchange

²⁶

L. lactis

NZ9800 (pNZ8037)

Expression vector with a nisin-inducible promoter, Cm^r

²⁵

NZ9800 (pNZ8037::isdB)

isdB gene cloned in pNZ8037 Cm^r

²⁶

E. coli

XL1-Blue

E. coli cloning host

Stratagene

BL21 (DE3)

E. coli cloning host

Invitrogen