Table 2 Abbreviations used as follows: AmpR, ampicillin resistance; KanR, kanamycin resistance.

From: Staphylococcus aureus iron-regulated surface determinant B (IsdB) protein interacts with von Willebrand factor and promotes adherence to endothelial cells

Plasmid

Feature

Marker

Reference

pQE30

E. coli vector for the expression of hexa-His-tagged recombinant proteins

AmpR

Qiagen

pQE30::isdB NEAT1-NEAT2

pQE30 encoding the NEAT1-NEAT2 domains of IsdB protein from S. aureus SH1000

AmpR

57

pQE30::isdB-NEAT1

pQE30 derivative encoding the NEAT1 domain of IsdB protein from S. aureus SH1000

AmpR

26

pQE30::isdB NEAT2

pQE30 derivative encoding the NEAT2 domain of IsdB protein from S. aureus SH1000

AmpR

26

pET28a

E. coli vector for the expression of hexa-His-tagged recombinant proteins

KanR

Millipore Sigma

pET28a::vWF-A1

pET28a derivative encoding the A1 domain of the human von Willebrand factor

KanR

This study