Figure 6 | Scientific Reports

Figure 6

From: Physiological significance of WDR45, a responsible gene for β-propeller protein associated neurodegeneration (BPAN), in brain development

Figure 6

Effects of Wdr45-knockdown on dendrite growth in vivo. (A) For sparse expression, pCAG-loxP-GFP (0.5 µg) was co-electroporated with pCAG-M-Cre (1 ng) together with pSuper-H1.shLuc (control), pSuper-Wdr45#1, #2 or pSuper-Wdr45#1 plus pCAG-Myc-Wdr45R (1.5 µg each of pSuper vector and 0.5 µg of pCAG-Myc-Wdr45R) at E14.5. Coronal sections were prepared, stained for GFP and analyzed at P10. Representative average z-stack projection images of layer II/III cortical neurons were shown. Scale bars, 50 μm. (B) Branch points of dendrites in (A) were quantified by Sholl test. One section from each brain was analyzed for control (n = 3), KD#1 (n = 7), KD#2 (n = 6), and KD#1 + Wdr45R (n = 5). Error bars indicate SD of the results from 10 to 15 neurons; ****p < 0.0001, by one-way ANOVA with Dunnett’s post hoc test (vs. control). (C,D) Total length was calculated for basal (C) and apical dendrites (D). #p < 0.05, ***p < 0.001 and ****p < 0.0001 (*vs control; #vs KD#1) by one-way ANOVA with Tukey’s post hoc test.

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