Figure 8 | Scientific Reports

Figure 8

From: Physiological significance of WDR45, a responsible gene for β-propeller protein associated neurodegeneration (BPAN), in brain development

Figure 8

Long-term effects of Wdr45-knockdown on dendrite growth in vivo. (A,B) For sparse expression, pCAG-loxP-GFP was co-electroporated with pCAG-M-Cre together with pSuper-H1.shLuc (control) (A) or pSuper-Wdr45#1 (KD#1) (B) at E14.5. After fixation at P30, P60 or P90, coronal sections were prepared and stained for GFP. Representative average z-stack projection images of layer II/III cortical neurons were shown. Scale bars, 50 μm. (C–E) Branch points of dendrites were analyzed by Sholl test. One section from each brain was analyzed for P30 (control, n = 10; KD#1, n = 11) (C), P60 (control, n = 6; KD#1, n = 8) (D) and P90 (control, n = 3; KD#1, n = 3). Error bars indicate SD of the results from 10 to 15 neurons; *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 by t-test (vs. control). (F,G) Total dendritic length was calculated for basal (F) as well as apical dendrites (G). Error bars indicate SD of the results from 10 to 15 neurons; *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 by t-test (vs. control).

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