Table 2 LOD robustness using heat-inactivated virus in pooled negative swab samples.

From: A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings

Viral copies/RDT

Positive test line

Positive control line

1.79 × 104

0/10

10/10

3.59 × 104

20/20

20/20

7.17 × 104

20/20

20/20

  1.  The dilution in bold (3.59 x 104 viral copies/RDT, 2.0 x 102 TCID50/mL) was the lowest detectable dilution in the series.
  2. SARS-CoV-2 RDT cassette replicates of both 7.17 × 104 viral copies/RDT and 3.59 × 104 viral copies/RDT had all 20/20 cassettes with positive test lines by visual inspection. For the 10 RDT cassettes to which 1.79 × 104 viral copies/RDT was applied, all 10/10 test lines were negative by visual inspection. The lowest level of virus where at least 95% of the replicates were detected was 3.59 × 104 viral copies/RDT.
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