Figure 6 | Scientific Reports

Figure 6

From: Nutritional evaluation and transcriptome analyses of short-time germinated seeds in soybean (Glycine max L. Merri.)

Figure 6

Expression pattern of genes involved in isoflavone metabolism. (A) Isoflavone pathway (details could be found in the Introduction section); (B) up and down regulated gene number between different germination time points (18HAG v.s. 0HAG, 36HAG v.s. 18HAG, 36HAG v.s. 0HAG); (C) Heatmap of coding genes expression pattern for IFS (isoflavone synthase), C4H (cinnamate 4-hydroxylase), FNS (flavone synthase), CHI (chalcone isomerase), DFR (dihydroflavonol 4-reductase), CHS (chalcone synthase), IF7GT/UGT (isoflavone 7-O-glucosyltransferase), PAL (phenylalanine ammonia-lyase), 4CL (4-coumarate-CoA ligase), F3H (flavanone 3-hydroxylase), 2HID (2-hydroxyisoflavanone dehydratase), CHR (chalcone reductase), MAT (isoflavone 7-O-glucoside 6"-O-malonyltransferase), IFR (isoflavone reductase); (D, E) qRT-PCR confirmation of representative genes UGT: Glyma.09G127700; IFR: Glyma.06G030900; MAT: Glyma.19G030500; 4CL: Glyma.14G223200) in isoflavone metabolic pathway. Relative expression level of each gene was normalized against Actin 11. *, **, ** indicates significant level of P < 0.05, 0.01, and 0.001 respectively by Student’s t-test, ns denotes none significant. Dashed line represents several steps.

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