Figure 2
From: Single-cell DNA and RNA sequencing of circulating tumor cells
Amount and quality of WGA sample. (a) Amounts of WGA samples obtained by each method. Method C (MDA-based) showed high productivity, but the amounts of WGA samples by method D (PCR with MDA hybridized) were too low to be evaluated by multiplex qPCR for QC. (b) Ct values of 8 cancer related genes, BRAF, EGFR, KIT, KRAS, NRAS, PIK3CA, PTEN, TP53 in WGA samples by each method. QC criteria was set as threshold cycles (Ct values) ≤ 30 in all 8 cancer related genes. In method A (PCR-based), seven of eight samples passed QC, in method B (MDA-based), all eight samples passed QC, and in method C (MDA-based), six of seven samples passed QC. In WGA samples assumed as low-quality, the Ct values of the 8 genes and their deviations were high.
