Figure 3 | Scientific Reports

Figure 3

From: Development of an advanced flow cytometry based high-resolution immunophenotyping method to benchmark early immune response in dairy cows

Figure 3

Gating strategy. (A) FSC-A vs. SSC-A, milk cells (A1), blood cells (A2); (B) Doublet discrimination, milk cells (B1) and blood cells (B2); (C) Determination of viability, single cells, milk (C1) and blood (C2); (D) Pan leukocyte marker CD45 vs. SSC-A, live cells, milk (D1) and blood (D2); (E1) Pan cytokeratin marker vs. SSC-A, CD45- cells, milk; (F) Autofluorescence (filter 525/50 nm) vs. SSC-A, granulocytes, milk (F1) and blood (F2); (G) CD11b vs. SSC-A, granulocytes, milk (G1) and blood (G2); (H) CD14 vs. CD16, monocytes/macrophages, milk (H1) and blood (H2); (I) gdTCR vs. CD335, lymphocytes, milk (I1) and blood (I2); (J) CD4 vs. CD8, rest1, milk (J1) and blood (J2); (K) CD21 vs. SSC-A, rest2, milk (K1) and blood (K2).

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