Figure 1
From: Mapping the electrostatic potential of the nucleosome acidic patch
Solution structure of the H2A-H2B dimer histone-fold core refined with NOE data. (a) Chemical shift indices based on experimental chemical shift Cα and Cβ (∆Cα–∆Cβ) for Dm. H2A-H2B. Secondary structure in the nucleosome crystal structure (PDB-id 2PYO) and the Hs. H2A-H2B solution structure (PDB-id 2RVQ) are indicated and labeled above the plot. (b) Strips from the 3D 15N-edited NOESY showing intermolecular NOEs between H2B amides (2H,-15N-labeled) and H2A side-chain protons (unlabeled). (c) Zoom on the H2A-H2B structure (PDB-id 2PYO) corresponding to the intermolecular contacts shown in the NOESY spectrum in panel (b) that restrain the H2B αC-helix position. (d) Superposition of the final ensemble of the Dm. H2A-H2B histone-fold core structure free in solution. (e) Overlay of the best scoring Dm. H2A-H2B dimer structure with the structure of the dimer in the nucleosome (PDB-id 2PYO). Acidic patch residues are shown as sticks in the best scoring model.
