Figure 2
Binding activity to human or cynomolgus CD25 and direct competitive binding characteristics of BA9 and BT942. (a) BA9 and BT942 specifically bind to SU-DHL-1 cells determined by flow cytometry. Data were presented as Mean ± SEM. (b) BA9 and BT942 specifically bind to HEK293T-CD25 cells determined by flow cytometry. Data were presented as mean ± SEM. (c,d) Binding kinetics of BA9 (c) or BT942 (d) for Human CD25 were measured in a surface plasmon resonance (SPR) assay with BIAcore. Experiments were performed in triplicate. (e,f) Cross-reactivity with cynomolgus CD25 by BA9 (e) and BT942 (f) were measured in a surface plasmon resonance (SPR) assay with BIAcore. Experiments were performed in triplicate. (g,h) Direct competitive binding characteristics of BA9 and BT942 was performed using in-tandem format binning assay. SA sensors immobilized with biotinylated human CD25 were saturated with the first antibody or PBST, then exposed to the second antibody or PBST. Data of KD were presented as mean ± SD.
