Figure 6

Comparison of CD25 expression level in human naïve CD4⁺ and CD8⁺ T, activated CD4⁺ and CD8⁺ T, Treg, iTreg cells and ADCC activity toward these different cell types by BT942. (a) Comparison of CD25 expression level in human naïve CD4⁺ and CD8⁺ T, activated CD4⁺ T and CD8⁺ T, Treg, iTreg cells. CD4⁺ and CD8⁺ T cells were activated by CD3/CD28 T Cell Activator for 48 h. CD4⁺, CD8⁺ naïve T cells and activated CD4⁺T, CD8⁺T cells were stained by anti-human CD4 or CD8 and CD25 fluorescent-labeled antibody. Treg and iTreg were stained by anti-human CD3, CD4, Foxp3 and CD25 fluorescent-labeled antibody. Cells were analyzed in flow cytometry by Beckman CytoFLEX. (b) ADCC activity toward these different cell types by BT942. The effector cell was PBMC.