Figure 1

ST09 induced cytotoxicity and apoptosis in ovarian cancer cell lines. (A) MTT assay of PA1 cells treated with increasing concentration of ST09 for 48 and 72 h. (B) MTT assay of A2780 cells treated with increasing concentration of ST09 for 48 and 72 h. (C) Dot plot and (D) Quantification of AnnexinV-FITC/PI double staining of PA1 cells treated for 48 h with increasing concentrations of ST09 analysed by flow cytometry. (E) Dot plot and (F) Quantification of AnnexinV-FITC/PI double staining of A2780 cells treated for 48 h with increasing concentrations of ST09 analysed by flow cytometry. Flow cytometry quantification depicts cell percentage in each quadrant plotted as means ± SD from three independent experiments. The quadrants A1 represents healthy or live cells (AnnexinV- and PI-), A2 represents late apoptotic cells (AnnexinV + and PI +), A3 represents dead cells (AnnexinV- and PI +), and A4 represents early apoptotic cells (AnnexinV + and PI-). Flow cytometry dot plots were generated using Gallios software (version 1.2, https://www.mybeckman.in/flow-cytometry/software/kaluza-for-gallios). Significance was plotted based on p values and represented as **(p-value < 0.01), ***(p-value < 0.001) and ****(p-value < 0.0001). The post-test for linear trend was significant with p-value < 0.0001 for the MTT assays in both cell lines for both time points.