Figure 2

Altered mitochondrial membrane potential and expression of mitochondrial-mediated apoptotic proteins in ST09 treated ovarian cancer cell lines. (A) Dot plot and (B) Quantification of JC1 staining of PA1 cells treated for 48 h with increasing concentrations of ST09 analysed by flow cytometry. (C) Dot plot and (D) Quantification of JC1 staining of PA1 cells treated for 48 h with increasing concentrations of ST09 analysed by flow cytometry. Flow cytometry quantification percentage of cells with green JC-1 monomers (apoptotic cells) and red JC1 aggregates (healthy cells). The median fluorescence intensity (MFI) was plotted as means ± SD from three independent experiments. Flow cytometry dot plots are generated using Gallios software (version 1.2, https://www.mybeckman.in/flow-cytometry/software/kaluza-for-gallios). (E) Apoptotic protein expression and (F) Quantification of PA1 cells treated with increasing concentrations of ST09 for 48 h. (G) Apoptotic protein expression and (H) Quantification of A2780 cells treated with increasing concentrations of ST09 for 48 h. Uncropped gel images are provided in the supplementary information. Significance plotted based on p values, and represented as *(p-value < 0.05), and ****(p-value < 0.0001).