Figure 5

ST09 downregulates downstream DDR1 target, MMPs inhibiting migration, wound closure and colony-forming properties in PA1 cells. (A) Expression of MMP proteins and (B) corresponding quantification in PA1 cells treated with increasing concentrations of ST09 for 48 h. (C) Expression of MMP proteins and (D) Corresponding quantification of A2780 cells treated with increasing concentrations of ST09 for 48 h. (E) Migration assay images and (F) corresponding quantification of the percentage migrated ST09-treated PA1 cells. Scale bar represents 200 µm (G) Wound healing assay images and (H) corresponding quantification representing percentage wound area at 0, 24 and 48 h time points of PA1 cells treated with increasing concentrations of ST09. Scale bar represents 200 µm (I) Clonogenic assay images and (J) corresponding quantification representing colony area percentage of PA1 cells treated with increasing concentrations of ST09. Uncropped gel images are provided in the supplementary information. All bar graphs are plotted as mean ± SD from three independent experiments. Significance was plotted based on p values, and represented as *(p-value < 0.05), **(p-value < 0.01), ***(p-value < 0.001) and ****(p-value < 0.0001).