Figure 6

ESR experimental spectrum of the 16-PCSL incorporated in gel membrane of DPPG (5 mM) in the presence of 0.1 mol% CTX (a, red line), the same spectrum shown in Fig. 5 h. The blue line corresponds to the best fitting of the theoretical simulation on the experimental data considering two components: the anisotropic site (b, Signal 1, green line) and a highly isotropic site (c, Signal 2, orange line). The proportion of the two components are indicated. The theoretical mobile component is very similar to the experimental spectrum obtained for 16-PCSL in solution in presence of the Crotoxin (d, wine line) and that of the pure probe 16-SASL in buffer PBS (e, violet line). The total spectra width is 100G.