Figure 2
From: The novel BET inhibitor UM-002 reduces glioblastoma cell proliferation and invasion
GBM cells proliferate and invade in a cerebral brain organoid-GBM co-culture model. (A) Total number of GBM cells at 1 and 9 days post co-culture; combined from three experimental replicates. (B) Representative maximum intensity projection images of fDISCO cleared cerebral brain organoid-GBM co-cultures. Top row: GFP-expressing GBM cells; bottom row: GFP merged with DAPI; scale bar for all images is in bottom right image and represents 300 µm. (C) Number of cells whose distance to the surface of the organoid exceeded the invasion threshold defined as the average third quartile value of the Day 9 group. (D) GBM cell distance to the nearest organoid surface. Boxplots represent individual organoids; data points are individual GBM cells. Distance was normalized by setting the minimum distance for each organoid to 0. (E) Normalized stacked kernel density estimation (KDE) of GBM cell distance to organoid surface. (F,G) Organoid volume (F) and surface area (G) measured using Imaris software. Statistical comparisons performed using an unpaired, two-tailed t test; **** ≤ 0.0001. Data from three independent biological replicates.
