Figure 5
From: The novel BET inhibitor UM-002 reduces glioblastoma cell proliferation and invasion
UM-002 induces changes in cell cycle related gene expression at single-cell resolution in vivo. (A) UMAP plot of the 38,200 total high-quality cell transcriptomes captured from both DMSO (gray) and UM-002 (green) treated PDX tumors. Cells are from tumors from 3 mice per treatment group. From DMSO treated mice, 18,452 cell transcriptomes were captured, while 19,748 were captured from UM-002 treated mice. (B,C) The GBM22 population of cells can be identified through analysis of individual cell percent alignment to hg19 (human) transcriptome (B), and percent alignment to mm10 (mouse) transcriptome. (C). (D) UMAP plot with pass-filter GBM22 cell transcriptomes (28,874 total GBM22, 14,878 DMSO treated, 13,996 UM-002 treated), colored by assigned cell cycle phase identity as determined using the R package Seurat52,53,54,55,59. (E) UMAP of GBM22 cell transcriptomes colored by G2M phase expression module score. (F) UMAP plot of GBM22 cell transcriptomes colored by S phase expression module score. (G) Connected bar plot showing the relative proportions of cells within G1, S, and G2M phases in DMSO and UM-002 treated tumors. (H) Bar plot showing changes in relative proportions (as percent) of cells within each phase of the cell cycle in UM-002 treated vs DMSO treated PDX tumors.
