Figure 1
Polymorphism rs2682818 is associated with down-regulation of miR-618 and the CRC risk. (a) Kaplan–Meier survival curve constructed for totally 205 colorectal cancer patients, who were divided into three groups according to the rs2682818 allele: homozygous (TT) genotype (n = 111) , homozygous (TG) genotype (n = 52) and homozygous (GG) genotype (n = 42). The survival data of each group were used to construct the K-M survival curve with CI = 95%, which can be further used as the historical reference survival curve. (b) In silico prediction of the secondary structure of different pre-miR-618 molecules. (c) q-PCR analysis of miR-618 in normal colon tissues and colorectal cancer tissues, in which U6 served as an internal control. Statistical analysis is done for groups with line, and the adjusted p value is represented above the line. ***P < 0.001 (t-test). (d) q-PCR analysis of miR-618 in colorectal cancer tissues which were divided into three groups, and U6 served as an internal control. Statistical analyses are done for groups with lines, and the adjusted p value are represented above the lines. *P < 0.05, **P < 0.01, ***P < 0.001 (t-test). (e) q-PCR analysis of miR-618 in different colorectal cancer cell lines, and U6 served as an internal control. (f) q-PCR analysis of miR-618 in 293T cells transfected with two kind of miR-618 expression plasmids, which were separately carried miR-168 precursor sequences harboring rs2682818 (SNP type) or without rs2682818 (wild type). *P < 0.05, **P < 0.01, ***P < 0.001 (t-test).
