Figure 6

Mono-association with A. tropicalis or a combination of bacteria with park²⁵ flies can alter eclosion rate. Embryos from park²⁵ flies were made axenic and then mono-associated with four different laboratory bacterial strains, or inoculated with an equal CFU combination of the four strains (Combo). Pupae count and eclosion was recorded from each vial. Heterozygous (Het) park²⁵ pupae were differentiated from the homozygous (Hom) park²⁵ pupae by the presence of the Tubby marker on the TM6C balancer chromosome. Data are presented as mean and SEM. Post-hoc Dunnett’s analysis comparing to the axenic flies are shown: **** = P < 0.0001, ** = P < 0.01, * = P < 0.05. Axenic (n = 160), L. brev = Lactobacillus brevis (n = 24), L. plant = Lactiplantibacillus plantarum (n = 37), A. pom = Acetobacter pomorum (n = 35), and A. trop = Acetobacter tropicalis (n = 37), combination (n = 32).