Table 1 Genotype and sex-dependent differences in microbiota composition of control and park²⁵ mutants.

	df	Weighted Unifrac					Unweighted Unifrac					Bray Curtis
	df	SS	MS	F	R²	P	SS	MS	F	R²	P	SS	MS	F	R²	P
G	2	0.37	0.19	72.18	0.71	0.001	0.20	0.10	6.12	0.21	0.001	6.71	3.36	111.90	0.79	0.001
S	1	0.00	0.00	1.38	0.01	0.24	0.02	0.02	1.25	0.02	0.29	0.04	0.04	1.33	0.00	0.25
V	3	0.04	0.01	5.02	0.07	0.002	0.04	0.01	0.90	0.05	0.54	0.47	0.16	5.27	0.06	0.001
GS	2	0.00	0.00	0.80	0.01	0.49	0.02	0.01	0.73	0.02	0.70	0.02	0.01	0.41	0.00	0.79
R	41	0.11	0.00	0.20			0.67	0.02	0.70			1.23	0.03	0.15
T	49	0.53	1.00				0.96	1.00				8.48	1.00

Effects of fly genotype (G), fly sex (S), fly vial (V), and the G-S interaction (GS) are shown along with residuals (R) and totals (T) as determined by PERMANOVA. PERMANOVA values are degrees of freedom (df), sum of squares (SS), mean squares (MS), F statistic (F), R² value (R²), and P-value (P).

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