Figure 4
From: Broad spectrum of CRISPR-induced edits in an embryonic lethal gene
PCR amplification of genomic DNA reveals larger deletions. A 1592 bp region spanning the third exon of the T gene was amplified from genomic DNA of 10 individual CRISPants (numbered 1 through 10) and genomic DNA from unmanipulated mice (Ctrl: control). The orange triangle identifies an amplicon of approximately 650 pb length in sample 2, indicative of a large deletion with an estimated length of 950 bp. Purple arrows point to bands in sample 3 of ~ 1.4 and ~ 1.3 kb size, respectively, indicating putative deletions of ~ 200 and ~ 300 bp size. Yellow arrows mark amplicons in sample 9 of ~ 1.3 kb and ~ 800 bp size, predicting the presence of deletions of ~ 300 bp and ~ 800 bp, respectively. M: Lane of marker DNA fragments of indicated sizes.
