Figure 1
From: A novel paper MAP method for rapid high resolution histological analysis
Tissue clearing and expansion via Tissue-MAP. (a) Schematic representation of Tissue-MAP protocol for clearing, expanding, and subsequent immunostaining of whole tissue. (b) Schematic representation of Paper-MAP processing using 100 μm-thick tissue sections. (c) Timeline of Tissue-MAP and Paper-MAP. (d) Optical transparency of injured mouse spinal cord achieved via Tissue-MAP. Tissue transparency was assessed against a patterned background (length:width = 5 mm:5 mm). (e and f) Three-dimensional rendering of lectin (red) and DiD-D (green) immunostaining of injured mouse spinal cord tissue processed via Tissue-MAP, imaged with light-sheet microscopy (range: 2000-μm) and confocal microscopy (tile-scanned single-z image). Dotted white lines indicate scar region. Scale bar, 100-μm. Illustration of mechanism is visualized by PowerPoint v2016.
