Figure 2
From: A novel paper MAP method for rapid high resolution histological analysis
Comparison of tissue clearing and expansion via optimized Paper-MAP. (a) Optical transparency of 100 μm-thick mouse brain sections processed via Paper-MAP optimization. (b) Effect of varying incubation times in low acrylamide (A.A) solution and Paper-MAP solution on tissue transparency, using 100 μm-thick rodent brain sections. (c) Comparison of gel size (width and length) before and after expansion, with varying incubation times in Paper-MAP solution. Quantification of total width and length from different ratio combinations. Data are presented as mean ± SD (standard deviation); n = 5 for each experimental group.
