Figure 3

Visualization of vasculature and neural structure in rodent brains via Paper-MAP. (a) SYTO-17 immunostaining in the hippocampus of mouse brain sections processed via Paper-MAP, imaged with confocal microscopy (4 × 5 panels, horizontal × vertical tiles). Each image (enlarged; i, ii, and iii) was taken with the same 63 × objective lens and z-stacked (range: 100-μm) for comparison. Scale bars (green, 1000-μm; gray, 100-μm). (b) Lectin and neurofilament (NF) immunostaining in the hippocampus and cortex of mouse brain sections processed via Paper-MAP. Each image of before (left; unexpanded mouse brain sections of 4% PFA fixed) was taken with the same 40 × objective lens and single z-images (5 × 5 panels, horizontal × vertical tiles) for comparison. Enlarged images are of each regions (yellow box). Images of after Paper-MAP (right) was taken with the same 40 × and 63 × objective lens and z-stacked (range: 100-μm) for comparison. 3D projection of the blood vessel (lectin, red) and neurofilament (green) focusing on the cortex and hippocampus regions, including the cornu ammonis 2 (CA2), dentate gyrus (DG). Scale bars (white, 500-μm; yellow, 50-μm). (c) Quantification of size in nucleus (blue) and blood vessel (red) of the before (unexpanded) and after Paper-MAP in mouse brain. Quantification of total diameter from different ratio combinations. Data are presented as mean ± SD (standard deviation); n = 5 for each experimental group. Illustration of mechanism is visualized by PowerPoint v2016.