Figure 5
From: A novel paper MAP method for rapid high resolution histological analysis
Paper-MAP processing of mouse spinal cord injury tissue. (a) Optical transparency of 100 μm-thick sections from mouse models of hemisection and deletion spinal cord injury models upon processing via Paper-MAP. Tissue transparency was assessed against a patterned background (length:width = 2 mm:2 mm). Representative images of samples from the hemisection spinal cord injury model processed via Paper-MAP and immunostained for lectin (red, range: 300-μm) (b), neurofilament (red) (c), and GFAP (red) (d). Yellow and white boxes indicate enlarged and 3D-projection images (range: 130-μm). Dotted white lines indicate scar region. Scale bars: white = 1000-μm, yellow = 100-μm. Illustration of mechanism is visualized by PowerPoint.
