Figure 8
From: A novel paper MAP method for rapid high resolution histological analysis
Application of Paper-MAP for tissue clearing and expansion in human submandibular gland organoid. (a) Schematic representation of Paper-MAP processing using 50 μm-thick human submandibular gland (SMG) organoid sections. (b) Optical transparency of human SMG organoid achieved via Paper-MAP. (c-e) Paper-MAP processing of human SMG organoids and subsequent immunostaining (range: 100-μm) for alpha-SMA (green), CK18 (red or green), NKCC1 (green), AQP5 (red) and DAPI (blue). Images of before (left, fixed in 4% PFA) and after Paper-MAP (right) was taken with the same 10 × and 40 × objective lens and z-stacked (range: 100-μm) for comparison. Scale bars: yellow = 50-μm. Illustration of mechanism is visualized by PowerPoint v2016.
